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Your Position: Home > Antibody > Doxorubicin > DON-MY2215

Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) (MALS verified)

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  • Source

    Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    1M2B1

  • Species

    Rabbit

  • Isotype

    Rabbit IgG | Rabbit Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Immunogen

    Doxorubicin-BSA.

  • Specificity

    Specifically recognizes Doxorubicin.

  • Application
    ApplicationRecommended Usage
    ELISA0.06-125 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Cross Verification

    This product can cross in Elisa with
    Doxorubicin.
    This product No cross-reactivity in ELISA with
    (PBD)SG3199.
    MMAF.
    Monomethyl auristatin E.
    Trastuzumab Biosimilar.

SDS-PAGE
Doxorubicin SDS-PAGE

Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Doxorubicin MALS images

The purity of Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) (Cat. No. DON-MY2215) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Doxorubicin ELISA

Immobilized Doxorubicin-ADC at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) (Cat. No. DON-MY2215) with a linear range of 0.06-8 ng/mL (QC tested).

  • Background
    Doxorubicin a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. As the payload of ADC drugs, Doxorubicin can act directly on DNA, insert into the double helix strands of DNA, cause the latter to unwind, change the template properties of DNA, and inhibit DNA polymerase, thereby inhibiting the synthesis of both DNA and RNA. Anti-Doxorubicin antibody is a rabbit monoclonal antibody specifically reacts with Doxorubin without other payloads, which is more sensitive than mouse antibody. The anti-Doxorubicin antibody is a useful reagent in PK assay to determine conjugated antibodies.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $315.00

Price(USD) : $1890.00

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