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Specifically recognizes PBD.
Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (1M1F9) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
1M1F9
Rabbit IgG | Rabbit Kappa
Unconjugated
PBD-BSA.
| Application | Recommended Usage |
| ELISA | 0.06-10000 ng/mL |
Protein A purified / Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Immobilized Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (1M1F9) (Cat. No. PAD-MY2212) at 1 μg/mL, add Loncastuximab Tesirine in the 100% Human Serum and then add Biotinylated Human CD19 (20-291), His,Avitag, premium grade (Cat. No. CD9-H82E9) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Serial dilutions of (PBD)SG3199 were added into Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (1M1F9) (Cat. No. PAD-MY2212)): Loncastuximab Tesirine binding reactions. The half maximal inhibitory concentration (IC50) is 0.02167 μg/mL (Routinely tested).
ELISA binding of Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (1M1F9) (Cat. No. PAD-MY2212) with Loncastuximab Tesirine, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 conjugated antibody respectively.
The coating antibody was Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (1M1F9) (Cat. No. PAD-MY2212), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including Loncastuximab Tesirine, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 conjugated antibodies used at 0.25 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-Payload PBD Antibody, Rabbit IgG (1M1F9) (Cat. No. PAD-MY2212) is specific to Loncastuximab Tesirine and has no cross-reactivity with IgG1-MMAF, Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 (Routinely tested).
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