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Your Position: Home > Kits > Fc epsilon RI alpha / IgE Fc > FRT-P008

Human Fc epsilon RI alpha / IgE Fc Inhibitor Screening Kit (TR-FRET)

For research use only.

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FAB
  1. Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
  2. Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
  3. Simple and fast operation - No complicated washing steps, significantly reducing time.
  4. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
  5. Accurate and reliable results - High sensitivity with minimal matrix effects.
  6. High throughput capability - Supports 500 tests, ideal for high-throughput screening.
  7. Fast completion - Results in just 1 hour.
    Product Specifications
    Assay TypeInhibition-TR-FRET
    AnalyteAnti-IgE Neutralizing Antibody
    Format100T/500T
    ReactivityHuman
    Regulatory StatusRUO
    SensitivityIC50=0.701 nM
    Standard Curve Range0.016 nM-66.6667 nM
    Assay Time1 hr
    Suitable Sample TypeFor human Fc epsilon RI alpha / IgE Fc Inhibitor Screening
    Sample volume10 μL
  • Product Overview
    The Human Fc epsilon RI alpha / IgE Fc Inhibitor Screening Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screening for inhibitors of human Fc epsilon RI alpha binding to human IgE Fc within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of human Fc epsilon RI alpha to bind to IgE Fc.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    FRTP008-C01Human IgE Fc Protein Europium-chelate100 tests/500 tests
    FRTP008-C02FA Labeled Human Fc epsilon RI alpha Protein100 tests/500 tests
    FRTP008-C03Anti-IgE (Omalizumab)30 μg/100 tests
    150 μg/500 tests
    DB-04TR-FRET Sample Dilution Buffer, pH7.450 mL/100 tests & 500 tests
    DB-05TR-FRET Detection Buffer, pH7.450 mL/100 tests & 500 tests
  • Assay Principles
    This Human Fc epsilon RI alpha / IgE Fc Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use a mixture of biotinylated human Fc epsilon RI alpha protein and FA labeled streptavidin as the acceptor and human IgE Fc protein Europium-chelate as the donor.
    — In the absence of inhibitors for human Fc epsilon RI alpha protein binding to human IgE Fc protein, the donor and acceptor are in close proximity due to the binding of human Fc epsilon RI alpha protein and human IgE Fc protein. Upon excitation with a specific light source, the donor emits a 620 nm signal, which is absorbed by the acceptor, resulting in a 665 nm emission.
    — In the presence of inhibitors that block the binding of human Fc epsilon RI alpha protein to human IgE Fc protein, the donor-acceptor interaction is disrupted, preventing FRET from occurring.

    Assay Principles

Bioactivity-TR-FRET Please refer to DS document for the assay protocol.
 Fc epsilon RI alpha / IgE Fc TR-FRET

Inhibition of Europium-chelate labeled Human IgE Fc: FA labeled human Fc epsilon RI alpha binding by Anti-IgE (Omalizumab)
Premix serial dilutions of Anti-IgE (Omalizumab) (1:2 serial dilution, from 10 μg/mL to 0.002 μg/mL (66.67-0.016 nM)) and Human IgE Fc Protein Europium-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA Labeled Human Fc epsilon RI alpha Protein and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 0.7155nM. The assay was performed according to the above-described Datasheet (QC tested).

  • Clinical and Translational Updates

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