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Your Position: Home > Antibody > FMC63 > FM3-AFM721

APC-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (0.03% Proclin)

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  • Source
    Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) is a neutralizing rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.
  • Application
    Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).
  • Clone
    1G10
  • Species
    Rabbit
  • Isotype
    Rabbit IgG | Rabbit Kappa
  • Specificity
    Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
  • Immunogen

    Recombinant FMC63 scFv derived from HEK293 cells

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Recommended Dilution
    1:50
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
Bioactivity-FACS
 FMC63 FACS

Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with APC-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (Cat. No. FM3-AFM721) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates

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