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This protein carries no "tag".
The protein has a calculated MW of 8.1 kDa. The protein migrates as 10 kDa±3 kDa under reducing (R) condition, and 15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human SDF-1 Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human SDF-1 Protein, premium grade (Cat. No. SD1-H5115) was greater than 95% as determined by SEC-HPLC.
Immobilized Human SDF-1 Protein, premium grade (Cat. No. SD1-H5115) at 5 μg/mL (100 μL/well) can bind Human CXCR4, Fc Tag (Cat. No. CX4-H5269) with a linear range of 1-39 ng/mL (QC tested).
Immobilized Human SDF-1 Protein, premium grade (Cat. No. SD1-H5115) at 5 μg/mL (100 μL/well) can bind Heparin Biotin with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Human SDF-1 Protein, premium grade (Cat. No. SD1-H5115) immobilized on CM5 Chip can bind Human CXCR4 Full Length Protein, Flag,His Tag (Cat. No. CX4-H52D3) with an affinity constant of 35.1 nM as determined in a SPR assay (in presence of Buffer B) (Biacore 8K) (Routinely tested).
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