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| Assay Type | FACS |
| Analyte | IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2 |
| Format | 50T |
| Reactivity | Mouse |
| Regulatory Status | RUO |
| Sensitivity | 1.53 pg/mL (IFN-γ), 1.18 pg/mL (TNF-α), 2.04 pg/mL (IL-10), 2.37 pg/mL (IL-6), 1.00 pg/mL (IL-4), 3.82 pg/mL (IL-2) |
| Standard Curve Range | 9.8-10000 pg/mL (IL-2, IL-6, IL-10, TNF-α, IFN-γ), 9.8-5000 pg/mL (IL-4) |
| Assay Time | 2.5 hr |
| Suitable Sample Type | Mouse serum |
| Sample volume | 30 μL |
Mouse Th1/Th2 Cytokine Kit (6-plex) (Flow Cytometry Multiplex Bead Assay, Non-Magbeads) gives quantitative results of f Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL10), Tumor Necrosis Factor α type (TNF-α) and Interferon-γ (IFN-γ) with a sample testing. The performance of this kit has been optimized for specific analysis of cytokines in mouse serum samples.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| Catalog | Product Name | Size |
| IL2-M51P3 | Mouse Th1/Th2 Standard | 5 μg |
| FMBc006-02 | Anti-mouse IL-2 Antibody-coupled APC-beads-B06 | 50 tests |
| FMBc005-02 | Anti-mouse IL-4 Antibody-coupled APC-beads-B05 | 50 tests |
| FMBc004-02 | Anti-mouse IL-6 Antibody-coupled APC-beads-B04 | 50 tests |
| FMBc003-02 | Anti-mouse IL-10 Antibody-coupled APC-beads-B03 | 50 tests |
| FMBc002-02 | Anti-mouse TNF-α Antibody-coupled APC-beads-B02 | 50 tests |
| FMBc001-02 | Anti-mouse IFN-γ Antibody-coupled APC-beads-B01 | 50 tests |
| FABr014-B | Biotinylated Monoclonal Anti-Mouse IL-2 Antibody, Rat IgG2a (AS541) | 0.25 mL |
| FABr015-B | Biotinylated Monoclonal Anti-Mouse IL-4 Antibody, Rat IgG1 (AS542) | 0.25 mL |
| FABr016-B | Biotinylated Monoclonal Anti-Mouse IL-6 Antibody, Rat IgG2a (AS543) | 0.25 mL |
| FABr017-B | Biotinylated Monoclonal Anti-Mouse IL-10 Antibody, Rat IgG2b (AS544) | 0.25 mL |
| FABr019-B | Biotinylated Monoclonal Anti-Mouse TNF-a Antibody, Rabbit IgG (006) | 0.25 mL |
| FABr018-B | Biotinylated Monoclonal Anti-Mouse IFN-γ Antibody, Rat IgG1 (AS545) | 0.25 mL |
| STN-NP119 | Streptavidin Protein-PE | 100 μg |
| FCMB-03 | Assay Buffer-01 | 50 mL |
| FCMB-04 | Wash Buffer-01 | 50 mL |
| FCMP-01 | 96-Well V-Bottom Assay Plate | 1 plate |
| FCMP-02 | 96-well Sealing film | 2 pieces |
| FMBf001-01 | APC Positive Control-01 | 0.5 mL |
| FMBf001-02 | PE Positive Control-01 | 0.5 mL |
A double logarithmic fitting curve was plotted for each cytokine ( IL-2,IL-4,IL-6, IL-10, TNF-α, IFN-γ). The present curve of each cytokines ( IL-2,IL-6, IL-10, TNF-α, IFN-γ) was ranging from 9.8 pg/mL to 10000 pg/mL respectively; The present curve of IL-4 was ranging from 9.8 pg/mL to 5000 pg/mL.
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on BD FACSymphony™ A1 Flow Cytometer, equipped with blue laser (561 nm) and red laser (637 nm).
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