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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9

Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) (MALS verified)

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  • Source

    Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    4D10

  • Species

    Mouse

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    virus

  • Immunogen

    Recombinant HSV-1 (strain 17) gH&gL Protein is expressed from human 293 cells

  • Specificity

    Specifically recognizes HSV-1 (strain 17) gH&gL Protein.

  • Application
    ApplicationRecommended Usage
    ELISA0.5-31 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein H & Glycoprotein L/gH & gL (HSV) SDS-PAGE

Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Glycoprotein H & Glycoprotein L/gH & gL (HSV) MALS images

The purity of Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) (Cat. No. GHL-MY2274) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Glycoprotein H & Glycoprotein L/gH & gL (HSV) ELISA

Immobilized HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-H5283) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain 17) gH&gL Antibody, Human IgG1 (4D10) (Cat. No. GHL-MY2274) with a linear range of 0.5-4 ng/mL (QC tested).

  • Background
    The herpes simplex virus type 1 (HSV-1) gH-gL complex which is found in the virion envelope is essential for virus infectivity and is a major antigen for the host immune system.The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $350.00

Price(USD) : $2310.00

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