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Your Position: Home > Protein > Annexin A5 > AN5-HF1H3

FITC-Labeled Human Annexin A5 Protein, His Tag

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  • Synonym
    ANXA5,ANX5,ENX2,PP4,Annexin V,Annexin A5
  • Source
    FITC-Labeled Human Annexin A5 Protein, His Tag(AN5-HF1H3) is expressed from E. coli cells. It contains AA Met 1 - Asp 320 (Accession # P08758-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Annexin A5 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 52.3 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Annexin A5 SDS-PAGE

FITC-Labeled Human Annexin A5 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-FACS
 Annexin A5 FACS

5e5 of Jurkat E6.1 cells were untreated or treated with 1ug/mL Ultra-LEAF Purified anti-human CD95 (Fas) Antibody (clone EOS9.1) followed by 5ug/mL of FITC-Labeled Human Annexin A5 Protein, His Tag (Cat. No. AN5-HF1H3). FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    Annexin A5 is a phospholipid binding protein, which binds with high affinity and selectivity to PS in the presence of calcium. PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin A5 will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Annexin A5 has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin A5 is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
  • Clinical and Translational Updates

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