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Biotin
1. Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain).
2. Immunoassay of bispecific antibody.
3. Beads coujugation for cell sorting or enrichment.
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
For long term storage, the product should be stored at liquid state at -70°C.
This product is stable after storage at:
Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), CF (carrier free), premium grade (Cat. No. G4S-BLM25A1) at 3 μg/mL (0.3 μg corresponds to labeling of 1e6 cells in a final volume of 100 µL) and PE-SA. PE signal was used to evaluate the binding activity (QC tested).
Non-specificity of Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), CF (carrier free), premium grade (Cat. No. G4S-BLM25A1) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), CF (carrier free), premium grade (0.3 μg corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
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