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Your Position: Home > Protein > IL-13 > IL3-H52H3

Human IL-13 Protein, His Tag, premium grade (cell activity verified)

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  • Synonym
    IL13, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13
  • Source
    Human IL-13 Protein, His Tag, premium grade(IL3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).
    Predicted N-terminus: Gly 21
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    IL-13 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 14.2 kDa. The protein migrates as 16 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method / rFC method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-13 SDS-PAGE

Human IL-13 Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-13 MALS images

The purity of Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H3) is more than 90% and the molecular weight of this protein is around 13-18 kDa verified by SEC-MALS.

Bioactivity-CELL BASE
 IL-13 CELL

Human IL-13 protein, His Tag, premium grade (Cat. No. IL3-H52H3) induced STAT6 Reporter activity in HEK293 cells. The EC50 value of this effect is 0.006870 μg/mL (Routinely tested).

Bioactivity-ELISA
 IL-13 ELISA

Immobilized Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H3) at 5 μg/mL (100 μL/well) can bind Human IL-13 R alpha 2 Protein, Fc Tag (Cat. No. IL2-H5256) with a linear range of 0.5-8 ng/mL (QC tested).

Bioactivity-SPR
 IL-13 SPR

Biotinylated Human IL-13 R alpha 1 Protein, His,Avitag (Cat. No. IL1-H82E8) immobilized on SA Chip can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H3) with an affinity constant of 13.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
 IL-13 BLI

Loaded Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) on Protein A Biosensor, can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H3) with an affinity constant of 17.2 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).

 IL-13 BLI

Loaded Monoclonal Anti-Human IL-13 Antibody, Human IgG4 on Protein A Biosensor, can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H3) with an affinity constant of 1.96 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).

  • Background
    Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.
  • Clinical and Translational Updates

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Price(USD) : $1980.00

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