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The kit is developed for quantitative detection of natural and recombinant human IL-7 in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | IL-5 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 1.0 pg/mL |
| Range | 1.95 pg/mL-250 pg/mL |
| Assay Time | 2.75 hr |
| Sample volume | 50 μL |
| Sample Type | For the quantitative determination of human IL-5 in serum, plasma and cell supernatant. |
| NIBSC Code | 90/586, NIBSC/WHO (90/586) approximate value (U/mL) = 0.075 x Clinmax Human IL-5 value (pg/mL) |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-5 is less than 1.0 pg/mL.
Ten replicates of each of three samples containing different IL-5 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of IL-5 were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recombinant IL-5 was spiked into three human serum samples, and then analyzed. The average recovery of IL-5 for serum samples is 99.7%.
| ID | Components | Size |
| CEA011-C01 | Pre-coated Anti-IL-5 Antibody Microplate | 1 plate |
| CEA011-C02 | Human IL-5 Standard | 100 ng×2 |
| CEA011-C03 | Biotin-Anti-IL-5 Antibody Con. Solution | 100 μL |
| CEA011-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA011-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA011-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA011-C07 | 20×Washing Buffer | 50 mL |
| CEA011-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA011-C09 | Substrate Solution | 12 mL |
| CEA011-C10 | Stop Solution | 6 mL |
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