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Your Position: Home > Immune Checkpoint Proteins > B7-H4 > Human B7-H4 /B7S1 /VTCN1 Protein

Human B7-H4 Protein

  • Synonym
    B7-H4,VTCN1,B7S1,B7h.5
  • Source
    Human B7-H4, His Tag (B74-H5222) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902).
    Predicted N-terminus: Phe 29
  • Molecular Characterization
    B7-H4(Phe 29 - Ala 258) NP_078902

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.1 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-H4, His Tag (Cat. No. B74-H5222) SDS-PAGE gel

Human B7-H4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human B7-H4, His TagHuman B7-H4, His Tag (Cat. No. B74-H5222) ELISA bioactivity

Immobilized Human B7-H4, His Tag (Cat. No. B74-H5222) at 2 μg/mL can bind anti-B7-H4 mAb (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).

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