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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Human full length PD1/PDCD1 Protein, Tag Free

Human PD-1 / PDCD1 Protein, Tag Free, Full Length

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Human PD-1, Tag Free, Full Length (PD1-HC214) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Leu 288 (Accession # NP_005009.2).
    N-terminal Sequence Analysis: Leu 25
  • Molecular Characterization
    PD-1(Leu 25 - Leu 288 ) NP_005009.2

    This protein carries no "tag".

    The protein has a calculated MW of 29.3 kDa. The protein migrates as 35-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with Detergent. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human PD-1, Tag Free, Full Length (Cat. No. PD1-HC214) SDS-PAGE gel

Human PD-1, Tag Free, Full Length on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
Human PD-1, Tag Free, Full LengthHuman PD-1, Tag Free, Full Length (Cat. No. PD1-HC214) ELISA bioactivity

Immobilized Human PD-1, Tag Free (Cat. No. PD1-HC214) at 0.2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L2, Fc Tag (Cat. No. PD2-H82F6) with a linear range of 1-39 ng/mL (QC tested).

Human PD-1, Tag Free, Full LengthHuman PD-1, Tag Free, Full Length (Cat. No. PD1-HC214) ELISA bioactivity

Immobilized Human PD-1, Tag Free, Full Length (Cat. No. PD1-HC214) at 0.2 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-L1, His Tag & Fc Tag (Cat. No. PD1-H82F3) with a linear range of 0.08-2.5 μg/mL (Routinely tested).

Human PD-1, Tag Free, Full LengthHuman PD-1, Tag Free, Full Length (Cat. No. PD1-HC214) ELISA bioactivity

Immobilized Human PD-1, Tag Free (Cat. No. PD1-HC214) at 0.2 μg/mL (100 μL/well) can bind Medarex with a linear range of 0.6-5 ng/mL (Routinely tested).

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