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Your Position: Home > MABSol® Biotin Labeled Proteins > CTLA-4 > Recombinant Cynomolgus CTLA-4 / CD152 / CELIAC3 / ICOS Protein, Biotinylated

Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avi Tag (Avitag™)

  • Synonym
    CTLA4,CD152,CELIAC3,GRD4,GSE,ICOS,IDDM12
  • Source
    Biotinylated Cynomolgus / Rhesus macaque CTLA-4 (CT4-C82E5) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    CTLA-4(Ala 37 - Ser 160) G7PL88

    This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus / Rhesus macaque CTLA-4 (Cat. No. CT4-C82E5) SDS-PAGE gel

Biotinylated Cynomolgus / Rhesus macaque CTLA-4 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity
Biotinylated Cynomolgus / Rhesus macaque CTLA-4Biotinylated Cynomolgus / Rhesus macaque CTLA-4 (Cat. No. CT4-C82E5) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avi Tag, His Tag (Cat. No. CT4-C82E5) with a linear range of 2-31 ng/mL (QC tested).

Biotinylated Cynomolgus / Rhesus macaque CTLA-4Biotinylated Cynomolgus / Rhesus macaque CTLA-4 (Cat. No. CT4-C82E5) ELISA bioactivity

Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque CTLA-4, Avi Tag, His Tag (Cat. No. CT4-C82E5) with a linear range of 2-63 ng/mL (Routinely tested).

  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $1380.00

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