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Multiplex Bead Assay Platform for Flow Cytometry

ACROBiosystems has independently developed a series of cytokine detection kits based on the Multiplex Bead Assay platform. Rigorously validated through comprehensive methodological studies, these assays enable accurate quantitative detection of single or multiple cytokines simultaneously using flow cytometry. Designed to support applications across preclinical research, clinical studies, and manufacturing quality control, they provide a comprehensive, efficient, and cost-effective solution to accelerate drug development workflows.

Additionally

Multiplex Bead Assay Principles：

1. Flow cytometry separates magnetic beads based on the fluorescent wavelength emitted by each bead. Specific antibodies are coupled to the surface of each bead which binds to a specific, different molecule.

2. PE-labeled detection antibodies are added to detect the molecules captured on the magnetic bead.

3. PE signal is detected by flow cytometry and quantified against a standard curve.

Multiplex Bead Assay Principles

Principle diagram

Product Advantages

Internationally Standardized: Calibrated against NIBSC/WHO international reference standards.

Low sample volume: 10-30 uL per sample.

Fast results: quantitatively evaluate multiple cytokines in a single experiment.

Less steps: easy-to-perform protocol with less steps avoids magnetic bead loss.

Wide detection range: 4 order magnitude standard curve.

Universally compatible: adaptable to various flow cytometers and software.

Protein signal-to-noise ratio was used to screen antibody pairs to ensure performance. Using antibodies with the required sensitivity, cell supernatant, serum, plasma and other samples were used to determine the specific recognition of antibodies to real samples

Antibody-pair

Magnetic beads are used as the carrier to bind antibodies to the magnetic beads through a chemical reaction. After coupling, antibody coupling amounts were evaluated for consistency between batches and validated.

Magnetic Beads

Non-specific signals from biotin and avidin are reduced by directly labeling by PE.

Detection Antibody

PBS is used to adjust the concentration to meet the interference level of common interfering substances in the kit samples, such as EDTA and hemoglobin.

Buffer System

Multiplex Cytokine Detection — Flexible Combination

More cytokine detection products

FAQ

Q: After multi-step magnetic separation, magnetic beads are starting to precipitate less and less. What should I do?

A: Slowly and carefully extract the supernatant during each cleaning. During adsorption, the magnetic absorption time of the well plate on the magnetic frame can be increased, and the magnetic beads should be obviously accumulated at the bottom.

Q: Only one board is provided. Can other wells be tested after one experiment?

A: It is recommended to cover the unused wells with foil or sealing film to avoid dust and other contamination.

Q: What if a large number of debris is observed in the FSC-SSC scatter-A diagram during data collection?

A: The FSC and SSC thresholds can be increased within reason.

Q: Flow cytometry has a wide detection range and does not require voltage adjustment. Is PE and APC compensation necessary for each sample?

A:Yes, the main purpose of sampling PE and APC compensation beads are to obtain appropriate fluorescence signals and ensure obvious clustering.

Q: How do you process the data?

A: FlowJo can be processed using FCAP, or exported with FCS, and processed with Graphpad.

  • Background
  • Product Advantages
  • Multiplex Cytokine Detection — Flexible Combination
  • More cytokine detection products
  • FAQ