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Product Details
Source
Polyclonal Olig2 Antibody, Rabbit IgG is a polyclonal antibody purified from rabbit serum.
Gene Synonyms: Oligo2, bHLHe19, oligodendrocyte transcription factor 2.Species
Rabbit polyclonal antibodies
Isotype
Rabbit IgG
Antibody Type
Polyclonal Antibody
Immunogen
Mouse Olig2 peptide fused to GST
Specificity
Usually, it is easier to get a positive signal in sections of juvenile animals than that of adults. This antibody does not recognize Olig1 and Olig3.
Application
ApplicationRecommended UsageIF1:200Purification
Protein A purified / Protein G purified
Formulation
Supplied as 0.2 μm filtered solution in PBS, 50 mM Glycine, 50 mM Tris with 40% glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Shipped at -20°C. Store at -20°C for 3 months;
- Shipped at -20°C. Store at -70°C for 12 months.
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Performance Data
Immunostaining
2D cell staining: Immunofluorescent staining (10X) of cerebral organoid-derived neurons (CIPO-BWL001K) labeling Olig2 (Red) with purified OL2-S456 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.
3D organoid staining: Immunofluorescent staining (4x or 10X) of cerebral organoid-(CIPO-BWL001K) labeling Olig2 (Green) with purified OL2-S456 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain. TUJ1 (red) is a neural differentiation marker commonly used to label neural processes.
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Background
Zhang K, et al. Nat Commun. 2022;13(1):1423. doi: 10.1038/s41467-022-29068-z.
Ono K, et al. Mol Cells. 2009;27(4):397-401. doi: 10.1007/s10059-009-0067-2.
General Notes: FOR RESEARCH USE ONLY.
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