>Immune Checkpoint Proteins
>Biotinylated Human PD-1 / PDCD1 Protein, Fc,Avitag,His Tag
This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags. The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100-130 kDa under non-reducing (NR) condition.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Human PD-1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 5 μg/mL (100 µl/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F2) with a linear range of 0.01-0.1 μg/mL (QC tested).
Serial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258): Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2966 μg/mL (Routinely tested).
Flow Cytometry assay shows that recombinant Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat No. PD1-H82F2) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 μg/mL (Routinely tested).
FACS analysis shows that the binding of Biotinylated Human PD-1, Fc,Avitag,His Tag to 293 overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-PD-L1 antibody. The concentration of PD-1 used is 1 μg/mL. The IC50 is 0.362 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $385.00
Price(USD) : $1518.00
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