Obesity Target Collection, Pioneer Therapeutic Innovation

Immobilized Human GLP-1R Full Length Protein, Flag,His Tag (Cat. No. GLR-H52D4) at 5 μg/mL (100 μL/well) on a Nickel Coated plate (Cat. No. SP-16) can bind Dulaglutide(a dual GLP-1R and GCGR agonist) with a linear range of 0.5-16 ng/mL.

Biotinylated Human GLP-1R, His,Avitag (Cat. No. GLR-H82E3) immobilized on CM5 Chip can bind GLP-1 (7-37) with an affinity constant of 1.33 μM as determined in a SPR assay (Biacore 8K).

Bioactivity analysis of human GLP-1R agonist (RLU). Human GLP-1R (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF096) was incubated with serial dilutions of Tirzepatide (a dual GLP-1R and GIPR agonist). The EC50 of Tirzepatide was approximately 1.11 nM.

cAMP accumulation assay for human GLP-1R agonist screening. HEK293/Human GLP-1R Stable Cell Line (Low Expression) (Cat. No. CHEK-ATP162), HEK293/Human GLP-1R Stable Cell Line (Medium Expression) (Cat. No. CHEK-ATP161) and HEK293/Human GLP-1R Stable Cell Line (High Expression) (Cat. No. CHEK-ATP160) were stimulated with Tirzepatide, respectively. The EC50 of Tirzepatide on HEK293/Human GLP-1R Stable Cell Line (High Expression) was approximately 0.0001544 μM.The EC50 of Tirzepatide on HEK293/Human GLP-1R Stable Cell Line (Medium Expression) was approximately 0.0005579 μM.The EC50 of Tirzepatide on HEK293/Human GLP-1R Stable Cell Line (Low Expression) was approximately 0.00136 μM.

Inhibition of human Activin E protein-induced reporter activity by anti-human ALK-7 neutralizing antibody. Human ALK-7 (Activin E receptor) (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF273) was incubated with serial dilutions of antibodies in the presence of human Activin E protein (Cat. No. INE-H5143) with a final concentration of 0.003 μg/mL. The EC50 of anti-human ALK-7 neutralizing antibody is approximately 0.2304 μg/mL.

Inhibition of human Activin E protein-induced reporter activity by human ALK-7 small molecule inhibitor. Human ALK-7 (Activin E receptor) (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF273) was incubated with serial dilutions of inhibitors in the presence of human Activin E protein (Cat. No. INE-H5143) with a final concentration of 0.003 μg/mL. The EC50 of human ALK-7 small molecule inhibitor was approximately 0.01536 μM.

NHP samples drug efficacy evaluation. NHP Serum Activin E Dimer Protein Levels was detected by Human Activin E Dimer ELISA Kit(Cat. No. CEA-B247) in the INHBE-siRNA Drug Treatment Group and the Control Group. Note:Data provided by third-party CRO, for reference only. Official validation shall be performed by the client.

LC/MS and ELISA methods consistency evaluation. Comparison LC/MS and Human Activin E Dimer ELISA Kit(Cat. No. CEA-B247) Methods to Measure Activin E Levels in Human serum. Note: Data sourced from ACRODx is for reference only. Specific validation requires re-verification by the customer.