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Your Position: Home > Receptor Tyrosine Kinases > PDGF R beta > Human PDGF R beta / CD140b Protein, His Tag

Human PDGF R beta / CD140b Protein, His Tag

  • Synonym
    PDGFRB,CD140B,JTK12,PDGF-R-beta,PDGFR-2,PDGFR-beta,PDGFR-β
  • Source
    Human PDGFRB, His Tag (PDB-H5220) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Phe 530 (Accession # AAH32224).
    Predicted N-terminus: Leu 33
  • Molecular Characterization
    Online(Leu 33 - Phe 530) AAH32224

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 57.0 kDa. The protein migrates as 95-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human PDGFRB, His Tag (Cat. No. PDB-H5220) SDS-PAGE gel

Human PDGFRB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human PDGFRB, His TagHuman PDGFRB, His Tag (Cat. No. PDB-H5220) ELISA bioactivity

Immobilized Human PDGF-BB, Tag Free (Cat. No. PDB-H4112) at 2 μg/mL (100 μL/well) can bind Human PDGFRB, His Tag (Cat. No. PDB-H5220) with a linear range of 0.039-0.625 μg/mL (QC tested).

  • Background
    Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB) is also called J03278, M21616, CD140B, JTK12, PDGF-R-beta and PDGFR, is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Recombinant soluble PDGFRB binds PDGF with high affinity and is potent PDGF antagonist. The ligands form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with –ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity, and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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