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Your Position: Home > Immune Checkpoint Proteins > CD28 > Mouse CD28 Protein, C-Fc Tag

Mouse CD28 Protein, Fc Tag

  • Synonym
    CD28,Tp44
  • Source
    Mouse CD28, Fc Tag (CD8-M5257) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # NP_031668.3).
    Predicted N-terminus: Asn 20
  • Molecular Characterization
    Online(Asn 20 - Lys 149) NP_031668.3

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 41.7 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CD28, Fc Tag (Cat. No. CD8-M5257) SDS-PAGE gel

Mouse CD28, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Mouse CD28, Fc TagMouse CD28, Fc Tag (Cat. No. CD8-M5257) ELISA bioactivity

Immobilized Mouse CD28, Fc Tag (Cat. No. CD8-M5257) at 5 μg/mL (100 μL/well)can bind Biotinylated Human B7-2, Fc Tag (Cat. No. CD6-H82F5) with a linear range of 0.156-2.5 μg/mL (QC tested).

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