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Your Position: Home > Receptor Tyrosine Kinases > Her2 > HE2-H525x

Human Her2 / ErbB2 (23-450) Protein, Fc Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Human Her2 (23-450), Fc Tag (HE2-H525x) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Ile 450 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
    Request for sequence
  • Molecular Characterization
    Online(Thr 23 - Ile 450) P04626-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 73.8 kDa. The protein migrates as 95-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

    Human Her2 (23-450), Fc Tag does not contain Herceptin MAb binding domain.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Her2 (23-450), Fc Tag (Cat. No. HE2-H525x) SDS-PAGE gel

Human Her2 (23-450), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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