Recombinant Human HER2 /ErbB2 (23-450) Protein, C-Fc Tag

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
Source
Human Her2 (23-450), Fc Tag (HE2-H525x) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Ile 450 (Accession # AAA75493).
Predicted N-terminus: Thr 23
Request for sequence
Molecular Characterization
rh HER2 / ErbB2 (23-450) Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 74.5 kDa. The predicted N-terminus is Thr 23. The reducing (R) protein migrates as 95-100 kDa in SDS-PAGE due to glycosylation.
rh HER2 / ErbB2 (23-450) Fc Chimera does not contain Herceptin mAb binding domain.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human Her2 (23-450), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Background
Human Epidermal growth factor Receptor 2 (HER2),also called ERBB2, HER-2,HER-2/neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.
References
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