0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Cytokines > IL-2 > IL2-H4113

Human IL-2 Protein, Tag Free

  • Synonym
    IL2,TCGF,lymphokine,Interleukin 2
  • Source
    Human IL-2, Tag Free (IL2-H4113) is expressed from E.coli cells. It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
    Predicted N-terminus: Ala 21
  • Molecular Characterization
    Online(Ala 21 - Thr 153) P60568-1

    This protein carries no "tag".

    The protein has a calculated MW of 15.4 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH5.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human IL-2, Tag Free (Cat. No. IL2-H4113) SDS-PAGE gel

Human IL-2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human IL-2, Tag FreeHuman IL-2, Tag Free (Cat. No. IL2-H4113) ELISA bioactivity

Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 2μg/mL (100 μL/well) can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with a linear range of 0.2-1.56 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) captured on CM5 chip via anti-human IgG Fc antibody, can bind IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 0.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human IL-2 R beta, His Tag (SPR verified) (Cat. No. CD2-H5221) on HIS1K Biosensor, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) on HIS1K Biosensor, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 18 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human IL-2, Tag Free (Cat. No. IL2-H4113) stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.284-0.355 ng/ml (Routinely tested).

  • Citations
  • Background
    Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

    Comments (0)

    Order Details

    Price(USD) : $195.00

    Price(USD) : $980.00

    Promotion & Exhibitions



    Drug Development Status

    • Number of Launched Drugs:2 Details
    • Number of Drugs in Clinical Trials:30 Details
    • Latest Research Phase:Approved

    Datasheet & Documentation

    Request for DMF
    DMF (Drug Master File)

    Contact Us

    +1  800-810-0816 (US)
    +86 400-682-2521 (AP)

    Questions & Comments

    This web search service is supported by Google Inc.

    totop