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Your Position: Home > Anti-idiotypic Antibody > Rituximab > Anti-Rituximab Antibodies

Anti-Rituximab Antibodies

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Rituximab F(ab')2.
  • Isotype
    IgG1/kappa
  • Specificity
    Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 years under sterile conditions after reconstitution.

SDS-PAGE
Anti-Rituximab Antibodies (Cat. No. RIB-Y35) SDS-PAGE gel

Anti-Rituximab Antibodies on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35) ELISA bioactivity

Anti-Rituximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized rituximab at 5 µg/ml, add increasing concentrations of Anti-Rituximab Antibodies (Cat. No. RIB-Y35, 10% human serum) and then add biotinylated rituximab at 5 µg/ml. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 20 ng/mL.

Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35) ELISA bioactivity

Demonstration of the specificity of Anti-Rituximab Antibodies (Cat. No. RIB-Y35) to the rituximab.

Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35) ELISA bioactivity

Detection of rituximab by anti-idiotypic capture ELISA in serum. Immobilized Anti-Rituximab Antibodies (Cat. No. RIB-Y35) at 0.5 μg/mL (100 μL/well), add increasing concentrations of rituximab (0.1% human serum). Detection was performed using  goat anti-human IgG  with a sensitivity of 0.156 μg/mL.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Rituximab Antibodies (mouse IgG1, Cat. No. RIB-Y35) captured on CM5 chip via anti-mouse antibodies surface, can bind Human rituximab with an affinity constant of 0.03 nM.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of rituximab to 293F overexpressing CD20 was inhibited by increasing concentration of Anti-Rituximab Antibodies (Cat. No. RIB-Y35). The concentration of rituximab used is 10 ng/ml. The IC50 is 0.013 μg/ml (Routinely tested).

  • Background
    Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $300.00

Price(USD) : $1980.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

Datasheet & Documentation

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