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Your Position: Home > Antibodies > Rituximab > RIB-Y35

Anti-Rituximab Antibodies

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Rituximab F(ab')2.
  • Isotype
  • Specificity
    Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 years under sterile conditions after reconstitution.
Anti-Rituximab Antibodies (Cat. No. RIB-Y35) SDS-PAGE gel

Anti-Rituximab Antibodies on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35) ELISA bioactivity

Anti-Rituximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized rituximab at 5 µg/mL, add increasing concentrations of Anti-Rituximab Antibodies (Cat. No. RIB-Y35, 10% human serum) and then add biotinylated rituximab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 20 ng/mL.

Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35) ELISA bioactivity

Demonstration of the specificity of Anti-Rituximab Antibodies (Cat. No. RIB-Y35) to the rituximab.

Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35) ELISA bioactivity

Detection of rituximab by anti-idiotypic capture ELISA in serum. Immobilized Anti-Rituximab Antibodies (Cat. No. RIB-Y35) at 0.5 μg/mL (100 μL/well), add increasing concentrations of rituximab (0.1% human serum). Detection was performed using goat Anti-Human IgG with a sensitivity of 0.156 μg/mL.


Anti-Rituximab Antibodies (mouse IgG1, Cat. No. RIB-Y35) captured on CM5 chip via anti-mouse antibodies surface, can bind Human rituximab with an affinity constant of 0.03 nM.

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of rituximab to 293F overexpressing CD20 was inhibited by increasing concentration of Anti-Rituximab Antibodies (Cat. No. RIB-Y35). The concentration of rituximab used is 10 ng/mL. The IC50 is 0.013 μg/mL (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

2e5 Anti-CD20 (Rituximab)-CAR 293 cells were first stained with Anti-Rituximab Antibodies (mouse IgG1, Cat. No. RIB-Y35) and followed by incubation with PE-labeled Anti-Mouse IgG1 antibody. PE-labeled Anti-Mouse IgG1 antibody was used as a negative control (Routinely tested).

  • Background
    Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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