>Anti-Trastuzumab Antibodies (recommended for PK/PD)
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Gly, 25mM Arg, 150 mM NaCl, pH7.5.. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 years under sterile conditions after reconstitution.
Anti-Trastuzumab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Detection of trastuzumab by bridging ELISA in serum. Immobilized Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1) at 2 μg/ml, added increasing concentrations of trastuzumab (10% human serum) and then added Biotinylated Anti-Trastuzumab Antibodies (Cat. No. TRB-BY5) at 2 μg/ml. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 4.8 ng/ml.
Demonstration of the specificity of Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1) to the trastuzumab.
Measured by its neutralizing ability in a functional ELISA. Immobilized trastuzumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1) and Biotinylated Human HER2 (Cat. No. HE2-H82E2) with a inhibition rate of 80%.
Anti-Trastuzumab Antibodies (mouse IgG1, Cat. No. TRB-Y1) captured on CM5 chip via anti-mouse antibodies surface, can bind human trastuzumab with an affinity constant of 0.296 nM.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $300.00
Price(USD) : $1980.00
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