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Your Position: Home > Antibodies > Trastuzumab > Anti-Trastuzumab Antibodies (recommended for PK/PD)

Anti-Trastuzumab Antibodies (recommended for PK/PD)

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Trastuzumab F(ab')2.
  • Isotype
    IgG1/kappa
  • Specificity
    Recognizes Trastuzumab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Gly, 25mM Arg, 150 mM NaCl, pH7.5.. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 years under sterile conditions after reconstitution.

SDS-PAGE
Anti-Trastuzumab Antibodies (recommended for PK/PD) (Cat. No. TRB-Y1) SDS-PAGE gel

Anti-Trastuzumab Antibodies (recommended for PK/PD) on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Anti-Trastuzumab Antibodies (recommended for PK/PD)Anti-Trastuzumab Antibodies (recommended for PK/PD) (Cat. No. TRB-Y1) ELISA bioactivity

Detection of trastuzumab by bridging ELISA in serum. Immobilized Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1) at 2 μg/ml, added increasing concentrations of trastuzumab (10% human serum) and then added Biotinylated Anti-Trastuzumab Antibodies (Cat. No. TRB-BY5) at 2 μg/ml. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 4.8 ng/ml.

Anti-Trastuzumab Antibodies (recommended for PK/PD)Anti-Trastuzumab Antibodies (recommended for PK/PD) (Cat. No. TRB-Y1) ELISA bioactivity

Demonstration of the specificity of Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1) to the trastuzumab.

Anti-Trastuzumab Antibodies (recommended for PK/PD)Anti-Trastuzumab Antibodies (recommended for PK/PD) (Cat. No. TRB-Y1) ELISA bioactivity

Measured by its neutralizing ability in a functional ELISA.  Immobilized trastuzumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1) and Biotinylated Human HER2 (Cat. No. HE2-H82E2) with a inhibition rate  of 80%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Trastuzumab Antibodies (mouse IgG1, Cat. No. TRB-Y1) captured on CM5 chip via anti-mouse antibodies surface, can bind human trastuzumab with an affinity constant of 0.296 nM.

  • Background
    Trastuzumab, is a monoclonal antibody used to treat breast cancer. Specifically it is used for breast cancer that is HER2 receptor positive. Trastuzumab works by binding to the HER2 receptor and slowing down cell duplication. Trastuzumab is a recombinant IgG1 kappa, humanized monoclonal antibody that selectively binds with high affinity in a cell-based assay (Kd = 5 nM) to the extracellular domain of the human epidermal growth factor receptor protein.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $300.00

Price(USD) : $1980.00

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