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Your Position: Home > Immune Checkpoint Proteins > PVRIG > PVG-H82F6

Biotinylated Human PVRIG Protein, Fc,Avitag™

  • Synonym
    C7orf15,C7orf15MGC138295,CD112R,MGC104322,MGC138297,MGC2463,PVRIG,CD112 receptor
  • Source
    Biotinylated Human PVRIG, Fc,Avitag (PVG-H82F6) is expressed from human 293 cells (HEK293). It contains AA Thr 41 - Asp 171 (Accession # Q6DKI7-1).
    Predicted N-terminus: Thr 41
  • Molecular Characterization
    Online(Thr 41 - Asp 171) Q6DKI7-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 41.9 kDa. The protein migrates as 46-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F6) SDS-PAGE gel

Biotinylated Human PVRIG, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human PVRIG, Fc,AvitagBiotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F6) ELISA bioactivity

Immobilized Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F6) with a linear range of 5-156 ng/mL (QC tested).

Biotinylated Human PVRIG, Fc,AvitagBiotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F6) ELISA bioactivity

Immobilized Human Nectin-2, His Tag (Cat. No. PV2-H52E2) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F6) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Biotinylated Human PVRIG, Fc,AvitagBiotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F6) ELISA bioactivity

Immobilized Human Nectin-2, Fc Tag (Cat. No. PV2-H5253) at 10 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F6) with a linear range of 0.01-0.156 μg/mL (Routinely tested).

  • Background
    Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The CD112R gene encodes a putative single transmembrane protein, which is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. Notably, the intracellular domain of phatases. The extracellular domain sequence of human and mouse CD112R have 65.3% similarity. CD112R may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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