>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Cetuximab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Detection of cetuximab by bridging ELISA in serum. Immobilized Anti-Cetuximab Antibodies (Cat. No. CEB-Y29) at 2 μg/mL, add increasing concentrations of cetuximab (10% human serum) and then add Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 12 ng/mL.
Demonstration of the specificity of Anti-Cetuximab Antibodies (Cat. No. CEB-Y29) to the cetuximab.
Measured by its neutralizing ability in a functional ELISA. Immobilized cetuximab at 2 μg/mL (100 μL/well) can bind pre-mixed Anti-Cetuximab Antibodies (Cat. No. CEB-Y29) and Biotinylated Human EGFRvIII, His Tag (Cat. No. EGR-H82E0) with a inhibition rate of 95%.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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