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Your Position: Home > Cytokines > IL-13 > Human IL-13 Protein, His Tag

Human IL-13 Protein, His Tag

  • Synonym
    IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13
  • Source
    Human IL-13, His Tag (IL3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).
    Predicted N-terminus: Gly 21
  • Molecular Characterization
    Online(Gly 21 - Asn 132) AAK53823.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 14.2 kDa. The protein migrates as 15 kDa and 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-13, His Tag (Cat. No. IL3-H52H4) SDS-PAGE gel

Human IL-13, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human IL-13, His TagHuman IL-13, His Tag (Cat. No. IL3-H52H4) ELISA bioactivity

Immobilized Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) at 2 μg/mL (100 μL/well) can bind Human IL-13, His Tag (Cat. No. IL3-H52H4) with a linear range of 5-39 ng/mL (QC tested).

Human IL-13, His TagHuman IL-13, His Tag (Cat. No. IL3-H52H4) ELISA bioactivity

Immobilized Human IL-13, His Tag (Cat. No. IL3-H52H4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-13 Antibody, Human IgG4 with a linear range of 0.3-5 ng/mL (Routinely tested).

  • Background
    Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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