>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Anti-Adalimumab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Detection of adalimumab by bridging ELISA in serum. Immobilized Anti-Adalimumab Antibodies (Cat. No. ADB-Y23) at 2 μg/mL, add increasing concentrations of adalimumab (10% human serum) and then add Biotinylated Anti-Adalimumab Antibodies (Cat. No. ADB-BY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 4.8 ng/mL.
Immobilized adalimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Adalimumab Antibodies (Cat. No. ADB-BY17) with a linear range of 0.78-25 ng/mL.
ELISA analysis shows that the binding of adalimumab to Human TNF-alpha, Tag Free, low endotoxin (Cat. No. TNA-H4211) was inhibited by increasing concentration of Biotinylated Anti-Adalimumab Antibodies (Cat. No. ADB-BY17). The concentration of adalimumab used is 6 ng/mL. The IC50 is 4.399 μg/mL.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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