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Your Position: Home > CD&BIOMARKER > Vitronectin > VIN-H8113

Biotinylated Human Vitronectin / VTN Protein, Tag Free, ultra sensitivity (primary amine labeling)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    VTN,V75,VN,Vitronectin,S-protein,Serum-spreading factor
  • Source
    Biotinylated Human Vitronectin, Tag Free, primary amine labeling (VIN-H8113) is expressed from E.coli cells. It contains AA Asp 20 - Arg 398 (Accession # P04004-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Asp 20 - Arg 398) P04004-1

    This protein carries no "tag".

    The protein has a calculated MW of 43.2 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Vitronectin, Tag Free, primary amine labeling (Cat. No. VIN-H8113) SDS-PAGE gel

Biotinylated Human Vitronectin, Tag Free, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human Vitronectin, Tag Free, primary amine labelingBiotinylated Human Vitronectin, Tag Free, primary amine labeling (Cat. No. VIN-H8113) ELISA bioactivity

Immobilized Biotinylated Human Vitronectin, Tag Free, primary amine labeling (Cat. No. VIN-H8113) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT5-H52W5) with a linear range of 0.156-1.25 μg/mL (QC tested).

  • Background
    Vitronectin is also known as S-protein, VN, VTN, V75. Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes. Blocking of Hic(a member of the pneumococcal surface protein C (PspC) family) by specific antiserum or genetic deletion significantly reduced pneumococcal binding to soluble and immobilised vitronectin and to Factor H, respectively. In addition, Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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