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Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit

For research use only.
  • Background
    The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health, and it spreads quickly. It is helpful to develop the Anti SARS-CoV-2 neutralizing antibody titer serologic assay kit to test the protective neutralizing antibody level in serum.
  • Application
    This kit is useful for serologic test for the titer of Anti-SARS-CoV-2 neutralizing antibody in serum/plasma in vitro.
    ELISA Buffer Set-96T (EBS-001) is sold separately and not included in kit, and please contact us if you need the buffer.
    It is for research use only.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store all items at -70℃. After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    No activity loss is observed after storage at: Room temperature (RT) for 1 month in lyophilized state; -20℃ for 1 year in lyophilized state; -70℃ for 6 months under sterile conditions after reconstitution.

  • Assay Principles
    This assay kit employs a competitive ELISA format and uniquely suitable for rapid high-throughput detecting the titer of Anti-SARS-CoV-2 neutralizing antibody in serum / plasma. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an Anti-SARS-CoV-2 neutralizing antibody (human IgG1) (as method verified Reference), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with SARS-CoV-2 S protein RBD.

    b) Add 50ul biotinylated human ACE2 to coated plate.

    c) Add the reference in kit or your samples in serum or plasma of interest.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the titer of your samples to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.

Typical Data
Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kitAnti-SARS-CoV-2 neutralizing antibody titer serologic assay kit (Cat. No. TAS-K003) ELISA bioactivity

Determination of Anti-SARS-CoV-2 Neutralizing Antibody titer in different concentrations of human serum.
Neutralizing titer of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat.No. SAD-S35) measured by Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit (Cat. No. TAS-K003) in different concentrations of human serum (CV < 30%).

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Order Details

Price(USD) : $480.00

Price(USD) : $2150.00

ELISA Buffer is not included in Inhibitor Screening Kit, please order ELISA Buffer Set if you need in your assay.

Price(USD) : $100.00

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Datasheet & Documentation

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DMF (Drug Master File)

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