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Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit

For research use only.
Materials Provided
IDComponentsSize(96 tests)Size (480 tests)
TAS003-C01SARS-CoV-2 S protein RBD10ug60ug
TAS003-C02Biotinylated Human ACE210ug10ug
SAD-S35Anti-SARS-CoV-2 neutralizing antibody20ug50ug
  • Background
    The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health, and it spreads quickly. It is helpful to develop the Anti SARS-CoV-2 neutralizing antibody titer serologic assay kit to test the protective neutralizing antibody level in serum.
  • Application
    This kit is useful for serologic test for the titer of Anti-SARS-CoV-2 neutralizing antibody in serum/plasma in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at-20℃. please store the plate and buffer products at 4-8℃ After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a competitive ELISA format and is uniquely suitable for rapid high-throughput detection of the titer of Anti-SARS-CoV-2 neutralizing antibody in serum / plasma. Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 Spike protein RBD, an Anti-SARS-CoV-2 neutralizing antibody (human IgG1) (as Reference), and Streptavidin-HRP reagent.

    a) Coat the plate with SARS-CoV-2 Spike protein RBD.

    b) Add 50ul biotinylated human ACE2 to coated plate.

    c) Add the reference in kit or your samples from serum or plasma of interest.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the titer of your samples to inhibit Spike protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.

Typical Data
Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kitAnti-SARS-CoV-2 neutralizing antibody titer serologic assay kit (Cat. No. TAS-K003) ELISA bioactivity

Determination of Anti-SARS-CoV-2 Neutralizing Antibody titer in different concentrations of human serum.
Neutralizing titer of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat.No. SAD-S35) mesured by SARS-CoV-2 inhibitor screening kit (Cat. No. EP-105) in different concentrations of human serum (CV < 30%).

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Order Details

Price(USD) : $480.00

Price(USD) : $2150.00

ELISA Buffer is not included in Inhibitor Screening Kit, please order ELISA Buffer Set if you need in your assay.

Price(USD) : $100.00

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