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Your Position: Home > Inhibitor screening kits > Spike RBD > RP-13

SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-Well

Materials Provided
ItemsSpecifications
MaterialPolystyrene
ColorClear
Plate Blocking:2% BSA Blocking Buffer
FormulationsClear, 96-well plates, coated with 100μL of Streptavidin tetramer, blocked with 300μL of 2% BSA Blocking Buffer and captured 0.1 μg/well of biotinylated SARS-CoV2 Spike RBD.
Detection MethodColorimetric
  • Background
  • Application
    This SARS-CoV2 Spike protein RBD- Coated Plate is intended for ELISA Assay and Biopannning.
  • Features
    1. Flexible experimental design because of the plate consist with detachable 8-well strips.

    2. Save your experiment time by pre-coated and blocked.

    3. No denaturing of the protein component of a conjugate upon binding.

    4. Ideal for binding anti-SARS2-CoV-2 S1 antibodies or ACE2 protein that typically exhibit poor binding to polystyrene.

  • Storage

    The unopened plate should be stored at 2°C to 8°C,The expiry date of the plate is 12 months. Once opened, place unused plates in a resealable bag with desiccant and store at 2°C to 8°C, The shelf life is 1 month from the date of opening.

  • Assay Principles
    Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. The SARS-CoV2 Spike protein RBD-Coated Plates are immobilized with biotinylated SARS-CoV2 Spike protein RBD to a Streptavidin tetramer protein coated plate, which is easy to use and widely available for applications.

    Example ELISA Procedure.

    Your experiment will include 4 simple steps:

    a) Add your antibody samples or ACE2 protein to the plate, incubation for 1 hour and wash the plate.

    b) Add a diluted Detection antibody to the plate and incubation.

    c) Wash the plate and next add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.

    Example Biopanning Procedure

    Biopanning is an affinity selection technique which selects for peptides that binds to a given target. In the capturing step, just add the antigen couple beads to your phage library. Simply use a magnet to separate the bound phages from the unbound ones. More efficient and time saving compared to the plate based capture.

Typical Data
SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-WellSARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-Well (Cat. No. RP-13) ELISA bioactivity

Immobilized biotinylated SARS-CoV2 Spike protein RBD at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind anti-SARS-CoV-2 S1 antibody with a linear range of 0.1-2 ng/mL (QC tested).

SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-WellSARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-Well (Cat. No. RP-13) ELISA bioactivity

Immobilized biotinylated SARS-CoV2 Spike protein RBD at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind ACE2 with a linear range of 0.2-4 ng/mL (QC tested).

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