|Plate Blocking:||2% BSA Blocking Buffer|
|Formulations||Clear, 96-well plates, coated with 100μL of Streptavidin tetramer, blocked with 300μL of 2% BSA Blocking Buffer and captured 0.1 μg/well of biotinylated SARS-CoV2 Spike protein RBD.|
Flexible experimental design because of the plate consist with detachable 8-well strips.
Save your experiment time by pre-coated and blocked.
No denaturing of the protein component of a conjugate upon binding.
Ideal for binding anti-SARS2-CoV-2 S1 antibodies or ACE2 protein that typically exhibit poor binding to polystyrene.
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. Plates are shipped at ambient temperature.
Example ELISA Procedure.
Your experiment will include 4 simple steps:
a) Add your antibody samples or ACE2 protein to the plate, incubation for 1 hour and wash the plate.
b) Add a diluted Detection antibody to the plate and incubation.
c) Wash the plate and next add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.
Example Biopanning Procedure
Biopanning is an affinity selection technique which selects for peptides that binds to a given target. In the capturing step, just add the antigen couple beads to your phage library. Simply use a magnet to separate the bound phages from the unbound ones. More efficient and time saving compared to the plate based capture.
Immobilized biotinylated SARS-CoV2 Spike protein RBD at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind anti-SARS-CoV-2 S1 antibody with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized biotinylated SARS-CoV2 Spike protein RBD at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind ACE2 with a linear range of 0.2-4 ng/mL (QC tested).
ACROBiosystems has developed a series of critical reagents for COVID-19 vaccine development and evaluation,including proteins, antibodies and kits. These featured products are suitable for IgG/M antibody titer detection, neutralizing antibody titer detection and antigen titer detection.
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