| ID | Composition | Size |
|---|---|---|
| TAS001-C01 | High-bind Plate | 96 tests |
| TAS008-C02 | SARS-CoV-2 Nucleocapsid protein | 30 μg |
| TAS008-C03 | Anti-SARS-CoV-2 Nucleocapsid Antibody (Control, IgG) | 10 μg |
| TAS001-C04 | HRP-Anti-Human IgG | 10 μg |
| TAS001-C05 | Blocking / Dilution Buffer | 60mL |
Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃.
It is recommended not to freeze thaw more than 3 times.
Your experiment will include 6 simple steps:
a) Coat the plate with Nucleocapsid Protein.
b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.
c) Add your sample to the plate, take the Anti-SARS-CoV-2 Nucleocapsid Antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.
d) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Nucleocapsid Protein at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody (Control, IgG) in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 6ng/mL. (QC tested).
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High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
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