|TAS010-C01||Anti-SARS-CoV-2 Nucleocapsid Antibody||60 μg|
|TAS008-C02||SARS-CoV-2 Nucleocapsid protein||10 μg|
|TAS010-C03||Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody||10 μg|
Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.
It is recommended not to freeze thaw more than 3 times.
Your experiment will include 6 simple steps:
a) Coat the plate with Anti-SARS-CoV-2 Nucleocapsid Antibody.
b) After washing and blocking, add your sample to the plate, take the SARS-CoV-2 Nucleocapsid protein as Control sample. The samples and Control sample are diluted by Lysis Buffer.
c) Add a diluted Secondary antibody biotin-anti-SARS-CoV-2 Nucleocapsid Antibody to the plate. The Secondary antody is diluted by Dilution Buffer.
d) Add a diluted Streptavidin-HRP to the plate.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
Detection SARS-CoV-2 Nucleocapsid Protein by ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein. And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody at 1:5000. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (QC tested).
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