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SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit

For research use only.
Materials Provided
TAS010-C01Anti-SARS-CoV-2 Nucleocapsid Antibody60 μg
TAS008-C02SARS-CoV-2 Nucleocapsid protein10 μg
TAS010-C03Biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody10 μg
TAS010-C04Streptavidin-HRP10 μg
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 protein in human.
  • Application
    This kit is developed for detecting the SARS-CoV-2 Nucleocapsid Protein in cell supernatant or SARS-CoV-2 virus in vitro.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Nucleocapsid Protein. The kit consists of Anti-SARS-CoV-2 Nucleocapsid Antibody, an SARS-CoV-2 Nucleocapsid Protein as Control, an biotin-Anti-SARS-CoV-2 Nucleocapsid Antibody and HRP-Streptavidin.

    Your experiment will include 6 simple steps:

    a) Coat the plate with Anti-SARS-CoV-2 Nucleocapsid Antibody.

    b) After washing and blocking, add your sample to the plate, take the SARS-CoV-2 Nucleocapsid protein as Control sample. The samples and Control sample are diluted by Lysis Buffer.

    c) Add a diluted Secondary antibody biotin-anti-SARS-CoV-2 Nucleocapsid Antibody to the plate. The Secondary antody is diluted by Dilution Buffer.

    d) Add a diluted Streptavidin-HRP to the plate.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.

Typical Data
SARS-CoV-2 Nucleocapsid Protein Titer Assay KitSARS-CoV-2 Nucleocapsid Protein Titer Assay Kit (Cat. No. TAS-K010) ELISA bioactivity

Detection SARS-CoV-2 Nucleocapsid Protein by ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein. And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody at 1:5000. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (QC tested).

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Price(USD) : $420.00

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