This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein RBD (A475V), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S protein RBD (A475V), His Tag (Cat. No. SPD-C52Hd) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-3 ng/mL (QC tested).
Serial dilutions of Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) were added into SARS-CoV-2 S protein RBD (A475V), His Tag (Cat. No. SPD-C52) : Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) binding reactions. The half maximal inhibitory concentration (IC50) is 19.5 μg/mL (Routinely tested).
Serial dilutions of Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat.No. SAD-S35) were added into SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag (Cat.No. SPD-C52Hd) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) : Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified) (Cat.No. AC2-H82E6) binding reactions. The half maximal inhibitory concentrations (IC50) of SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag (Cat.No. SPD-C52Hd) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) are 4.67291 μg/mL and 1.44469 μg/mL respectively (Routinely tested).
Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (Cat. No. SPD-M128) were added into SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag (Cat.No. SPD-C52Hd) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) : Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified) (Cat.No. AC2-H82E6) binding reactions. The half maximal inhibitory concentrations (IC50) of SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag (Cat.No. SPD-C52Hd) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) are 0.26478 μg/mL and 0.78328 μg/mL respectively (Routinely tested).
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (A475V), His Tag (Cat. No. SPD-C52Hd) with an affinity constant of 25.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (A475V), His Tag (Cat. No. SPD-C52Hd) with an affinity constant of 87.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD (A475V), His Tag (Cat. No. SPD-C52Hd) with an affinity constant of 11 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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