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Your Position: Home > Isotype Controls > IgG1 Fc > IG1-H82Eb

Biotinylated Human IgG1 Fc Protein, Avitag™,His Tag (MALS-verified)

  • Synonym
    IgG1
  • Source
    Biotinylated Human IgG1 Fc, Avitag,His Tag (IG1-H82Eb) is expressed from human 293 cells (HEK293). It contains AA Pro 100 - Lys 330 (Accession # P01857-1(C103S)).
    Predicted N-terminus: Pro 100
  • Molecular Characterization
    Online(Pro 100 - Lys 330) P01857-1 (C103S)

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 28.7 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl,pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IgG1 Fc, Avitag,His Tag (Cat. No. IG1-H82Eb) SDS-PAGE gel

Biotinylated Human IgG1 Fc, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human IgG1 Fc, Avitag,His Tag (Cat. No. ) MALS images

The purity of Biotinylated Human IgG1 Fc, Avitag,His Tag (Cat. No.IG1-H82Eb) was more than 90% and the molecular weight of this protein is around 55-66 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human IgG1 Fc, Avitag,His TagBiotinylated Human IgG1 Fc, Avitag,His Tag (Cat. No. IG1-H82Eb) ELISA bioactivity

Immobilized Recombinant Protein A at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag,His Tag (Cat. No. IG1-H82Eb) with a linear range of 0.2-3 ng/mL (QC tested).

  • Background
    Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $150.00

    Price(USD) : $3000.00

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    Datasheet & Documentation

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    DMF (Drug Master File)

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