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Your Position: Home > Isotype Controls > IgG2a Fc > IGA-M8216

Biotinylated Mouse IgG2a Fc protein, Avitag™ (MALS verified)

  • Synonym
    IgG2A
  • Source
    Biotinylated Mouse IgG2a Fc, Avitag™ (IGA-M8216) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # P01863-1).
    Predicted N-terminus: Glu 98
  • Molecular Characterization
    Online(Glu 98 - Lys 330) P01863-1

    This protein carries an Avi tag at the C-terminus.

    The protein has a calculated MW of 38.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse IgG2a Fc, Avitag™ (Cat. No. IGA-M8216) SDS-PAGE gel

Biotinylated Mouse IgG2a Fc, Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Mouse IgG2a Fc, Avitag™ (Cat. No. ) MALS images

The purity of Biotinylated Mouse IgG2a Fc, Avitag™(Cat. No. IGA-M8216) was more than 90% and the molecular weight of this protein is around 57-67 kDa verified by SEC-MALS.

  • Background
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $1380.00

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    Datasheet & Documentation

    Request for DMF
    DMF (Drug Master File)

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