|TAS019-C01||High-bind Plate||96 tests|
|TAS019-C02||SARS-CoV-2 Spike RBD||30ug|
|TAS019-C03||Anti-SARS-CoV-2 Antibody (Control, Monkey IgG)||10ug|
|TAS019-C04||HRP-Goat anti-Monkey IgG||10ug|
|TAS019-C05||Blocking / Dilution Buffer||60mL|
Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃.
It is recommended not to freeze thaw more than 3 times.
Your experiment will include 6 simple steps:
a) Coat the plate with SARS-CoV-2 Spike protein RBD.
b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.
c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.
d) Add diluted Secondary antibody HRP-Goat-Anti-Monkey IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
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