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Your Position: Home > Antibody titer assay kits > Spike RBD > TAS-K019

Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit

For research use only.
This product is still under development. Please contact us if you have interest in this product. We will accelerate the development process accordingly and reserve this product for you as request.
Materials Provided
IDComponentsSize
TAS019-C01High-bind Plate96 tests
TAS019-C02SARS-CoV-2 Spike RBD30ug
TAS019-C03Anti-SARS-CoV-2 Antibody (Control, Monkey IgG)10ug
TAS019-C04HRP-Goat anti-Monkey IgG 10ug
TAS019-C05Blocking / Dilution Buffer60mL
  • Background
    The ongoing SARS-COV-2 pandemics has sickened 3.7 million people around the world, claiming over 1,000,000 lives. To deal with this global public health crisis, unprecedented efforts are being made to study the virus, SARS-CoV-2. At present, there are 193 new crown vaccine projects registered with the World Health Organization are being developed. To support these studies, ACRO has developed Monkey Anti-SARS-CoV-2 antibody IgG Serologic Assay kit.
  • Application
    This kit is developed for serologic test for Monkey IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state;
    Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Monkey serum by SARS-CoV-2 Spike RBD. The Kit consists of High-bind Plate, Spike RBD, an Anti-SARS-CoV-2 Antibody (Control, Monkey IgG), an HRP-Anti-Goat anti monkey IgG secondary antibody.

    Your experiment will include 6 simple steps:

    a) Coat the plate with SARS-CoV-2 Spike protein RBD.

    b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.

    c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.

    d) Add diluted Secondary antibody HRP-Goat-Anti-Monkey IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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Price(USD) : $600.00

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