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Your Position: Home > All Other Proteins > NKG2D > NKD-H82E3

Biotinylated Human NKG2D / CD314 Protein, His,Avitag™

  • Synonym
    NKG2D,CD314,KLRK1,NK cell receptor D
  • Source
    Biotinylated Human NKG2D, His,Avitag (NKD-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 73 - Val 216 (Accession # P26718-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Ile 73 - Val 216) P26718-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 20.2 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human NKG2D, His,Avitag (Cat. No. NKD-H82E3) SDS-PAGE gel

Biotinylated Human NKG2D, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human NKG2D, His,AvitagBiotinylated Human NKG2D, His,Avitag (Cat. No. NKD-H82E3) ELISA bioactivity

Immobilized Human MICA, Fc Tag (MALS verified) (Cat. No. MIA-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human NKG2D, His,Avitag (Cat. No. NKD-H82E3) with a linear range of 0.078-1.250 μg/mL (QC tested).

Biotinylated Human NKG2D, His,AvitagBiotinylated Human NKG2D, His,Avitag (Cat. No. NKD-H82E3) ELISA bioactivity

Immobilized Biotinylated Human NKG2D, His,Avitag (Cat. No. NKD-H82E3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human MICA, Fc Tag (MALS verified) (Cat. No. MIA-H5253) with a linear range of 0.2-2 ng/mL (Routinely tested).

  • Background
    NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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  • Latest Research Phase:Phase 2 Clinical

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