This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.8 kDa.
It is produced as active membrane protein with full ECD epitopes, and contains both the ECL1 and ECL2.
>60% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Immobilized Biotinylated Human Claudin-18.2, His,Avitag (Cat. No. CL2-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (in presence of DDM and CHS) (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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