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Your Position: Home > All Other Proteins > Claudin-18.2 > CL2-H82E3

Biotinylated Human Claudin-18.2 Protein, His,Avitag™ (active membrane protein)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    Claudin-18.2,CLDN18,Claudin-18
  • Source
    Biotinylated Human Claudin-18.2, His,Avitag (CL2-H82E3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Met 1 - Ala  200) P56856-2

    This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.8 kDa.
    It is produced as active membrane protein with full ECD epitopes, and contains both the ECL1 and ECL2.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >60% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.

    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.

    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
Biotinylated Human Claudin-18.2, His,AvitagBiotinylated Human Claudin-18.2, His,Avitag (Cat. No. CL2-H82E3) ELISA bioactivity

Immobilized Biotinylated Human Claudin-18.2, His,Avitag (Cat. No. CL2-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (in presence of DDM and CHS) (QC tested).

  • Background
    Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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