0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > Claudin-18.2 > CL2-H5546

Human Claudin-18.2 Protein, His Tag (active membrane protein)

  • Synonym
    Claudin-18.2,CLDN18,Claudin-18
  • Source
    Human Claudin-18.2, His Tag (CL2-H5546) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Met 1 - Ala 200) P56856-2

    This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.0 kDa.
    It is produced as active membrane protein with full ECD epitopes, and contains both the ECL1 and ECL2.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >60% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep Claudin-18.2 soluble and active, under no circumastance should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
Bioactivity-ELISA
Human Claudin-18.2, His TagHuman Claudin-18.2, His Tag (Cat. No. CL2-H5546) ELISA bioactivity

Immobilized Human Claudin-18.2, His Tag (Cat. No. CL2-H5546) at 2 μg/mL (100 μL/well) on an Nickel Coated plate can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.4-2 ng/mL (in presence of DDM and CHS) (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human Claudin-18.2, His Tag (Cat. No. CL2-H5546) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with an affinity constant of 3.55 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 on AHC Biosensor, can bind Human Claudin-18.2, His Tag (Cat. No. CL2-H5546) with an affinity constant of 1.55 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $850.00

Price(USD) : $3000.00

Price(USD) : $7200.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:18 Details
  • Latest Research Phase:Phase 3 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop