This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.0 kDa.
It is produced as active membrane protein with full ECD epitopes, and contains both the ECL1 and ECL2.
>60% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep Claudin-18.2 soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Claudin-18.2, His Tag (Cat. No. CL2-H5546) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with an affinity constant of 3.55 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Loaded Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 on AHC Biosensor, can bind Human Claudin-18.2, His Tag (Cat. No. CL2-H5546) with an affinity constant of 1.55 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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