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Your Position: Home > Other Coronavirus Proteins > Spike protein > SPN-M52H4

MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    MERS Spike protein trimer, His Tag (SPN-M52H4) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K9N5Q8-1). The recombinant protein has a T4 fibritin trimerization motif. Proline substitutions (V1060P, L1061P) and alanine substitutions (R748A, R751A) are introduced to stabilize the trimeric prefusion state of MERS-CoV Spike protein and abolish the furin cleavage site, respectively.
    Predicted N-terminus: Tyr 18
  • Molecular Characterization
    Online(Tyr 18 - Trp 1295) K9N5Q8-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 145.9 kDa. The protein migrates as 150 kDa and 175-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H4) SDS-PAGE gel

MERS Spike protein trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

MERS Spike protein trimer, His Tag (Cat. No. ) MALS images

The purity of MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H4) is more than 90% and the molecular weight of this protein is around 540-580 kDa verified by SEC-MALS.

MERS Spike protein trimer, His TagMERS Spike protein trimer, His Tag (Cat. No. SPN-M52H4) ELISA bioactivity

Immobilized MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H4) at 5 μg/mL (100 μL/well) can bind Human DPPIV, Fc Tag (Cat. No. DP4-H5266) with a linear range of 0.1-2 ng/mL (QC tested).

MERS Spike protein trimer, His TagMERS Spike protein trimer, His Tag (Cat. No. SPN-M52H4) ELISA bioactivity

Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 μg/mL (100 μL/well) can bind MERS Spike protein trimer, His Tag (Cat. No. SPN-M52H4) with a linear range of 0.1-3 ng/mL (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

  • Purchaser Notification
    Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).
  • Limited Use Label License No. 17: Prefusion Coronavirus Spike Proteins
    This product and its use is the subject of one or more U.S. Provisional Patent Application Nos. 62/412,703; 62/972,886; U.S. Patent Application No. 16/344,774 and corresponding patents and applications in other countries. This product is sold under licenses from The National Institute of Allergy & Infectious Diseases/National Institutes of Health. Rights to use this product are limited to research only (including Non-clinical Diagnostic Kits), and expressly exclude the right to use in human therapeutics, vaccines and clinical diagnostics and any use involving the administration of this product to humans.

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