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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > CT4-H82A3

Biotinylated Human CTLA-4 Protein, Mouse IgG2a Fc,Avitag™

  • Synonym
    CTLA4,CD152
  • Source
    Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (CT4-H82A3) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # Q6GR94-1).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    Online(Ala 37 - Phe 162) Q6GR94-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 42.1 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (Cat. No. CT4-H82A3) SDS-PAGE gel

Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Humane CTLA-4, Mouse IgG2a Fc,AvitagBiotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (Cat. No. CT4-H82A3) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (Cat. No. CT4-H82A3) with a linear range of 0.3-5 ng/mL (QC tested).

Biotinylated Humane CTLA-4, Mouse IgG2a Fc,AvitagBiotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (Cat. No. CT4-H82A3) ELISA bioactivity

Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (Cat. No. CT4-H82A3) with a linear range of 0.3-2 ng/mL (Routinely tested).

Biotinylated Humane CTLA-4, Mouse IgG2a Fc,AvitagBiotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (Cat. No. CT4-H82A3) ELISA bioactivity

Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag (Cat. No. CT4-H82A3) with a linear range of 0.2-2 ng/mL (Routinely tested).

  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:32 Details
  • Latest Research Phase:Approved

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