This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 51.8 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>85% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human GPRC5D, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Anti-GPRC5D Antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human GPRC5D, Flag,His Tag (Cat. No. GPD-H52D3) with an affinity constant of 71 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Loaded Anti-GPRC5D Antibody, Human IgG4 on Protein A Biosensor, can bind Human GPRC5D, Flag,His Tag (Cat. No. GPD-H52D3) with an affinity constant of 12.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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