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Your Position: Home > All Other Proteins > Claudin-18.2 > CL2-H5547

Human Claudin-18.2 Full Length Protein-VLP (HEK293)

  • Synonym
    Claudin-18.2,CLDN18,Claudin-18
  • Source
    Human Claudin-18.2 Full Length Protein-VLP (CL2-H5547) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 261 (Accession # P56856-2).
    Predicted N-terminus: Met
  • Molecular Characterization

    This protein carries no "tag". The protein has a calculated MW of 27.7 kDa.

    Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane proteins (like Claudin-18.2) can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

    The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of ~150 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    Intact particles captured by Electron Microscope.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;

    2. -70°C for 3 months under sterile conditions.

  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
Bioactivity-ELISA
Human Claudin-18.2 Full Length Protein-VLPHuman Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-H5547) ELISA bioactivity

Immobilized Human Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-H5547) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.8-3 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human Claudin-18.2 Full Length Protein-VLP (Cat. No. CL2-H5547) captured on CM5 Chip via Anti-Claudin-18.2 antibody can bind Anti-Claudin-18.2 antibody with an affinity constant of 1.24 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.
  • Clinical and Translational Updates

      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:18 Details
  • Latest Research Phase:Phase 3 Clinical

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