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HRP-SARS-CoV-2 Spike RBD (L452R, T478K), His Tag

  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
  • Source
    HRP-SARS-CoV-2 Spike RBD, His Tag (SPD-SR2M4) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(L452R, T478K)).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Arg 319 - Lys 537 ) QHD43416.1 (L452R, T478K)

    The protein has a calculated MW of 67.8 kDa.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
HRP-SARS-CoV-2 Spike RBD, His TagHRP-SARS-CoV-2 Spike RBD, His Tag (Cat. No. SPD-SR2M4) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind HRP-SARS-CoV-2 Spike RBD, His Tag (Cat. No. SPD-SR2M4) with a linear range of 0.004-0.125μg/mL (QC tested).

HRP-SARS-CoV-2 Spike RBD, His TagHRP-SARS-CoV-2 Spike RBD, His Tag (Cat. No. SPD-SR2M4) ELISA bioactivity

Detection of HRP-SARS-CoV-2 Spike RBD, His Tag titer by compitive-ELISA Assay.Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M122) were added into Human ACE2 / ACEH Protein, Fc Tag (Cat. No. AC2-H525): HRP-SARS-CoV-2 Spike RBD, His Tag (Cat. No. SPD-SR2M4) binding reactions. The half maximal inhibitory concentration (IC50) is 0.105 μg/mL (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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