Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins (mp, GPRC5D) and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution. The GPRC5D nanodisc assembles from a mixture of GPRC5D protein in detergent, phospholipid micelles and membrane scaffold protein (MSP1D1) upon removal of the detergent.
This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag with calculated MW of 52.5 kDa . The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa.
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