This protein carries no "tag".
The protein has a calculated MW of 18.3 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-18, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-18, Tag Free (Cat. No. IL8-H5113) is more than 90% and the molecular weight of this protein is around 15-21 kDa verified by SEC-MALS.
Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Human IL-18 R1, Fc Tag (Cat. No. IL1-H5259) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 0.409 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) on Protein A Biosensor, can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 8.09 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Human IL-18 R1, Fc Tag (Cat. No. IL1-H5259) on Protein A Biosensor, can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 179 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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