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Your Position: Home > Cytokines > IL-18 > IL8-H5113

Human IL-18 Protein, Tag Free (MALS verified)

  • Synonym
    IL-18,Interleukin-18,IL18,IL-1 gamma,IGIF,IL1F4,IL-1F4
  • Source
    Human IL-18, Tag Free (IL8-H5113) is expressed from E.coli cells. It contains AA Tyr 37 - Asp 193 (Accession # Q14116-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Tyr 37 - Asp 193) Q14116-1

    This protein carries no "tag".

    The protein has a calculated MW of 18.3 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-18, Tag Free (Cat. No. IL8-H5113) SDS-PAGE gel

Human IL-18, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IL-18, Tag Free (Cat. No. ) MALS images

The purity of Human IL-18, Tag Free (Cat. No. IL8-H5113) is more than 90% and the molecular weight of this protein is around 15-21 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Human IL-18 R1, Fc Tag (Cat. No. IL1-H5259) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 0.409 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) on Protein A Biosensor, can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 8.09 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Human_FcRn_Heterodimer_Protein_Bli

Human IL-18 R1, Fc Tag (Cat. No. IL1-H5259) on Protein A Biosensor, can bind Human IL-18, Tag Free (Cat. No. IL8-H5113) with an affinity constant of 179 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin-18 (IL-18) is a potent proinflammatory cytokine that induces interferon-gamma (IFN-gamma) production from Th1 cells, NK cells and activated macrophages, particularly in the presence of IL-12. IL-18 also functions in developmental regulation of T-lymphocyte helper type I cells and in Fas-mediated cytotoxicity. Suppression of IL-18 activity is being investigated for treatment of chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. It acts by inducing heterodimerization of the two subunits of its receptor, IL-18RAlpha and IL-18RBeta shows structural similarity to IL-1.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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