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Your Position: Home > Receptor Tyrosine Kinases > VEGF R1 > VE1-H5220

Human VEGF R1 / Flt-1 Protein, His Tag

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
FLT,VEGFR1,FLT1
Source
Human VEGF R1, His Tag (VE1-H5220) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Asn 756 (Accession # NP_002010.1).
Predicted N-terminus: Ser 27
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 83.3 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human VEGF R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA

Human VEGF R1, His TagHuman VEGF R1, His Tag (Cat. No. VE1-H5220) ELISA bioactivity

Immobilized Human VEGF165, Tag Free (Cat. No. VE5-H4210) at 0.01 μg/mL (100 μL/well) can bind Human VEGF R1, His Tag (Cat. No. VE1-H5220) with a linear range of 4-31 ng/mL (QC tested).

Protocol

Citations
- Methods of producing glycosylated proteins
  Authors: ML Wetter, et al
  Journal: US20180354997A1 43447
  Application: SPR
  Request for Full-text
- A biosynthetic route for polysialylating proteins in Escherichia coli
  Authors: Keys TG, et al.
  Journal: Metab Eng 2017
  Application: SPR
  Request for Full-text
Background
Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.
References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.