This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 83.3 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human VEGF R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Loaded Human VEGF R1, His Tag (Cat. No. VE1-H5220) on HIS1K Biosensor, can bind ActiveMax® Human VEGF165, Tag Free (Cat. No. VE5-H4210) with an affinity constant of 43.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: ML Wetter, et al
Journal: US20180354997A1 2018
Authors: Keys TG, et al.
Journal: Metab Eng 2017
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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