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Your Position: Home > Enzyme > Cas12a > CAA-L5140

GENPower™ NLS-Cas12a Nuclease (MALS verified)

  • Synonym
    CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, cas12a, Cpf1
  • Source
    GENPower™ NLS-Cas12a Nuclease(CAA-L5140) is expressed from E.coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58 ).
    Predicted N-terminus: Met
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as 135-145 kDa under reducing (R) condition (SDS-PAGE). This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Concentration
    10ug/ul.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM NaAC,500 mM NaCl,0.1 mM EDTA,0.1 mM TCEP,pH 6.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 3 months under sterile conditions.
SDS-PAGE
Cas12a SDS-PAGE

GENPower™ NLS-Cas12a Nuclease on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cas12a MALS images

The purity of GENPower™ NLS-Cas12a Nuclease (Cat. No. CAA-L5140) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

Bioactivity
 Cas12a ENZYME

Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage (QC tested).

  • Background
    Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium,Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/RNA(crRNA) molecules into mature crRNAs which allows the possibility to use both mature or pre-crRNA for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering tool. CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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