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Your Position: Home > All Other Proteins > MMP-2 > Human MMP-2 Protein, His Tag

Human MMP-2 Protein, His Tag

  • Synonym
    MMP2,CLG4,CLG4A,MMP-II,MONA,TBE-1
  • Source
    Human MMP-2, His Tag (MM2-H5225) is expressed from human 293 cells (HEK293). It contains AA Ala 30 - Cys 660 (Accession # AAH02576).
    Predicted N-terminus: Ala 30
  • Molecular Characterization
    Online(Ala 30 - Cys 660) AAH02576

    Human MMP-2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 71.8 kDa. The predicted N-terminus is Ala 30. The reducing (R) protein migrates as 66-71 kDa in SDS-PAGE .

    Pre-activation is required for enzymatic assays. Please dilute Human MMP-2 to 50-100 µg/mL in TCNB buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5), and then add p-aminophenylmercuric acetate (APMA, Sigma, Catalog # A-9563) to a final concentration of 1 mM. Please keep the enzyme with APMA for 2-6 hour at 37°C. Please note that the optimal treatment time may need to be determined empirically. *100mM APMA stock solution should be prepared in DMSO. Please avoid adding high concentration APMA solution (>20 mM) directly into the reaction as it tends to precipitate. A pre-dilution step is highly recommended.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human MMP-2, His Tag (Cat. No. MM2-H5225) SDS-PAGE gel

Human MMP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

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